Journal
SCIENCE
Volume 307, Issue 5706, Pages 124-127Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1103631
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Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as kiss-and-run was unrelated to transmitter release. The release-related capacitance. change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine, 5'-triphosphate (GTP) analog GTPgammaS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.
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