Journal
NEUROSCIENCE LETTERS
Volume 373, Issue 2, Pages 105-109Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2004.09.070
Keywords
human teratocarcinoma NTera2/cloneD1 cells; retinoic acid; neuronal differentiation; basic helix-loop-helix (bHLH) transcription factors; neuronal markers
Categories
Ask authors/readers for more resources
Human teratocarcinoma NTera2/cloneD1 (NT2) cells are able to generate postmitotic neurons in response to retinoic acid (RA) and for this reason these cells provide an important tool to study human neurogenesis in vitro. We have obtained neurons by treating NT2 aggregated cells with RA for solely 14 days. RT-PCR assays showed that NT2 cells express mRNAs of several neural bHLH genes such as Hes1, Ngn1, Mash1, NeuroD, Math1 and Pax6. just in the early days of RA exposure. In particular, we reported for the first time that RA treatment was followed by a modulation of endogenous Ngn1 and Math1 transcripts. RT-PCR and Western blotting experiments also demonstrated expression of typical neuronal markers such as GluR. MAP2, Tau and NeuN. Knowledge of the expression pattern of the different neuronal genes during! NT2 commitment could be used to investigate alterations in the molecular pathways involved in the human neuronal differentiation. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available