Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 72B, Issue 1, Pages 37-42Publisher
WILEY
DOI: 10.1002/jbm.b.30119
Keywords
microbial transglutaminase; gelatin; scaffold; hydrogel; tissue engineering
Funding
- Engineering and Physical Sciences Research Council [GR/S21755/01] Funding Source: researchfish
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The definitive goal of this research is to develop protein-based scaffolds for use in soft tissue regeneration, particularly in the field of dermal healing. The premise of this investigation was to characterize the mechanical properties of gelatin cross-linked with microbial transglutaminase (mTGase) and to investigate the cytocompatibility of mTGase cross-linked gelatin. Dynamic rheological analysis revealed a significant increase in the storage modulus and thermal stability of gelatin after cross-linking with mTGase. Static, unconfined compression tests showed an increase in Young's modulus of gelatin gels after mTGase cross-linking. A comparable increase in gel strength was observed with 0.03% mTGase and 0.25% glutaraidehyde cross-linked gelatin gels. In vitro studies using 3T3 fibroblasts indicated cytotoxicity at a concentration of 0.05% mTGase after 72 h. However, no significant inhibition of cell proliferation was seen with cells grown on lower concentrations of mTGase cross-linked gelatin substrates. The mechanical improvement and cytocompatibility of mTGase crosslinked gelatin suggests mTGase has potential for use in stabilizing gelatin gels for tissueengineering applications. (C) 2004 Wiley Periodicals, Inc.
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