Glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline

A sensitive and selective amperometric glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline (PANI) was investigated. Poly (m-phenylenediamine) (PMPD), which was employed as an anti-interferent barrier and a protective layer to platinum microparticles, was deposited onto platinum-modified PANI in the presence of glucose oxidase. The morphology of PANI, Pt/PANI and PMPD-GOD/Pt/PANI were investigated by scanning electron microscopy. The results show that PANI has a nano-fibrous morphology. The enzyme electrode exhibits excellent response performance to glucose with linear range from 2 x 10(-6) to 12 x 10(-3) M and fast response time within 7 s. Due to the selective permeability of PMPD, the enzyme electrode also shows good anti-interference to uric acid and ascorbic acid. The Michaelis-Menten constant k(m) and the maximum current density i(max) of the enzyme electrode were 9.34 x 10(-6) M and 917.43 muA cm(-2), respectively. Furthermore, this glucose biosensor also has good stability and reproducibility. (C) 2004 Elsevier B.V. All rights reserved.