Journal
BMC NEUROLOGY
Volume 5, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2377-5-1
Keywords
-
Categories
Funding
- NINDS NIH HHS [P01-NS44155, K24-NS02091, P01 NS044155, K24 NS002091] Funding Source: Medline
- PHS HHS [R01-27713] Funding Source: Medline
Ask authors/readers for more resources
Background: The primary aim of this study is to demonstrate the feasibility of utilizing doxycycline to suppress matrix metalloproteinase-9 (MMP-9) in brain arteriovenous malformations (AVMs). Methods: Ex-vivo treatment of AVM tissues: Intact AVM tissues were treated with doxycycline for 48 hours. Active and total MMP-9 in the medium were measured. Pilot trial: AVM patients received either doxycycline (100 mg) or placebo twice a day for one week prior to AVM resection. Active and total MMP-9 in BVM tissues were measured. Results: Ex-vivo treatment of AVM tissues: Doxycycline at 10 and 100 mu g/ml significantly decreased MMP-9 levels in AVM tissues ex-vivo (total: control vs 10 vs 100 mu g/ml = 100 +/- 6 vs 60 +/- 16 vs 61 +/- 9%; active: 100 +/- 8 vs 48 +/- 16 vs 59 +/- 10%). Pilot trial: 10 patients received doxycycline, and 4 patients received placebo. There was a trend for both MMP-9 levels to be lower in the doxycycline group than in the placebo group (total: 2.18 +/- 1.94 vs 3.26 +/- 3.58, P = .50; active: 0.48 +/- 0.48 vs 0.95 +/- 1.01 ng/100 mu g protein, P = .25). Conclusions: A clinically relevant concentration of doxycycline decreased MMP-9 in ex-vivo AVM tissues. Furthermore, there was a trend that oral doxycycline for as short as one week resulted in a decrease in MMP-9 in AVM tissues. Further studies are warranted to justify a clinical trial to test effects of doxycycline on MMP-9 expression in AVM tissues.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available