Journal
CELL METABOLISM
Volume 1, Issue 2, Pages 93-106Publisher
CELL PRESS
DOI: 10.1016/j.cmet.2004.12.009
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Krox20 is a zinc finger-containing transcription factor that is abundantly expressed in adipose tissue. However, its role in fat cell differentiation has not been established. In cultured 3T3-L1 cells, Krox20 is rapidly induced by serum stimulation. Overexpression of Krox20 in both 3T3-L1 preadipocytes and multipotent NIH3T3 cells promotes adipogenesis in a hormone-dependent manner. Conversely, RNAi-mediated loss of Krox20 function reduced adipogenesis in 3T3-L1 cells. Ectopic expression of Krox20 can transactivate the C/EBP promoter and increase C/EBP beta gene expression in 3T3-L1 preadipocytes. RNAi-mediated knockdown of C/EPB beta diminished Krox20's proadipogenic effect. Finally, coexpression of Krox20 and C/EBP beta in naive NIH3T3 cells resulted in the pronounced induction of a fully differentiated adipocyte phenotype, an effect previously observed only with PPAR gamma. These data indicate that Krox20 is necessary for adipogenesis and that, when overexpressed, Krox20 potently stimulates adipogenesis via C/EBP beta-dependent and -independent mechanisms.
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