Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 31, Issue 2, Pages 155-160Publisher
SPRINGER
DOI: 10.1007/s10858-004-7912-6
Keywords
complex; nitroxide; NMR; spin-label; TEMPO
Categories
Ask authors/readers for more resources
Nitroxide species, which have an unpaired electron localized on a nitrogen atom, can be useful as NMR probes to identify areas of the surface of a protein involved in the formation of a complex. The proximity of an electron spin leads to higher NMR relaxation rates for protein nuclei. If a protein-ligand complex is formed the radical is excluded from certain sites on the protein surface, protecting them from relaxation effects. We show here that charged nitroxide species can be helpful for identifying regions of the surface of the (4)F1(5)F1 module pair from human fibronectin involved in peptide binding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available