Journal
PLANT SCIENCE
Volume 168, Issue 2, Pages 487-491Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2004.09.012
Keywords
tanshinones; isoprenoid pathways; elicitors; Salvia miltiorrhiza; hairy root culture
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This work examined the accumulation of diterpenoid tanshinones and related secondary metabolism pathways in hairy root cultures of Salvia miltiorrhiza Bunge (Lamiacae) induced by a biotic elicitor, the carbohydrate fraction of yeast extract (YE, 100 mug mL(-1)), and an abiotic elicitor, Ag+ (30 muM). The activity of 3-hydroxy-3-methylglutaryl CoA redutase (HMGR) was only stimulated by Ag+, and 1-deoxyD-xylulose 5-phosphate synthase (DXS) was stimulated by both, but more strongly by YE. While the non-MVA pathway inhibitor fosmidomycin inhibited the tanshinone accumulation induced by both elicitors, the MVA-pathway inhibitor mevinolin only suppressed the A-induced tanshinone accumulation. The results suggest that the tanshinone accumulation induced by the two elicitors was mainly synthesized via the non-MVA pathway (DXS activity), but could depend on crosstalk between the MVA and non-MVA pathways. Furthermore, the pretreatment of hairy roots with Ag+ for 24-48 h potentiated the YE-induced tanshinone production and DXS activity. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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