Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 140, Issue 2, Pages 219-231Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2004.10.006
Keywords
cyanobacterium; Electrospray ionization mass spectrometry; glycolipids lipid analysis; phosphatidylglycerol; platelet aggregation; platelet activating; factor; Scytonema julianum; sphingosine
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Cyanobacteria serve as a rich source of novel bioactive metabolites. Few studies on glycolipids reported them as having specific biological activities. In this study, total lipids of Scytonema julianum, a filamentous cyanobacterium isolated from a Greek cave, were separated into neutral and phospho- and glycolipids, and the latter were further fractionated by high-performance liquid chromatography (HPLC). Each glycolipid fraction was tested in vitro for its ability to inhibit platelet-activating factor (PAF)- and thrombin-induced washed rabbit platelet aggregation and/or to cause platelet aggregation. The structures of the most active fractions were elucidated by biological assays, by chemical determinations and identified by electrospray mass spectrometry. One fraction was a potent inhibitor of PAF-induced platelet aggregation. Structural studies of this fraction indicated the existence of a phosphoglyco-analog of acyl-sphingosine. Two fractions causing platelet aggregation were detected and identified as phosphoglycolipids. The first one -,vas identified as a phosphoelyco-analog of acyl-acetylated sphingosine and the second one as a glyco-analog of phosphatidylglycerol. The presence of the above bioactive compounds demonstrates new types of lipids in cyanobacteria in regard to the structure and biological activity In addition. the identified bioactive lipids may contribute to the allergic character of cyanobacteria. (C) 2004 Elsevier Inc. All rights reserved.
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