Journal
PLANT MOLECULAR BIOLOGY
Volume 57, Issue 3, Pages 435-444Publisher
SPRINGER
DOI: 10.1007/s11103-004-0076-7
Keywords
Arabidopsis; Integrase; nuclear localization; phage HK022; site-specific recombination
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The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP x attB) as well as a site-specific excision reaction (attL x attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.
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