Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 16, Issue 2, Pages 689-700Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E04-09-0828
Keywords
-
Categories
Funding
- NIGMS NIH HHS [R01 GM053498, GM-53498] Funding Source: Medline
- NINDS NIH HHS [R01 NS040929, R01NS40929] Funding Source: Medline
Ask authors/readers for more resources
The Trc/Ndr/Saxi/Cbk1 family of ser/thr kinases plays a key role in the morphogenesis of polarized cell structures in flies, worms, and yeast. Tricornered (Trc), the Drosophila nuclear Dbf2-related (Ndr) serine/threonine protein kinase, is required for the normal morphogenesis of epidermal hairs, bristles, laterals, and dendrites. We obtained in vivo evidence that Trc function was regulated by phosphorylation and that mutations in key regulatory sites resulted in dominant negative alleles. We found that wild-type, but not mutant Trc, is found in growing hairs, and we failed to detect Trc in pupal wing nuclei, implying that in this developmental context Trc functions in the cytoplasm. The furry gene and its homologues in yeast and Caenorhabditis elegans have previously been implicated as being essenhal for the function of the Ndr kinase family. We found that Drosophila furry (Fry) also is found in growing hairs, that its subcellular localization is dependent on Trc function, and that it can be coimmunoprecipitated with Trc. Our data suggest a feedback mechanism involving Trc activity regulates the accumulation of Fry in developing hairs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available