Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes)

We present cytogenetic analyses of four fish species, belonging to four Loricariidae subfamilies: Neoplecostomus microps (Neoplecostominae) with 2n = 54 chromosomes, Harttia loricariformis (Loricariinae) with 2n = 56 chromosomes, Hypostomus affinis ( Hypostominae) with 2n = 66 chromosomes and Upsilodus sp. (Upsilodinae), with 2n = 96 chromosomes. In addition to karyotypes, data on the location of 18s rDNA sites are presented, derived from indirect ( silver nitrate impregnation) and direct ( FISH) methods. There is only one pair of nucleolar organizing regions (NORs) per species, except in H. affinis. Diversity and NOR macrokaryotypic evolution in the species analyzed are discussed in relation to the evolution of the Loricariidae as a whole. In addition, a revision of the cytogenetic data available for this family is presented.