Journal
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Volume 111, Issue 2, Pages 181-192Publisher
SPRINGER
DOI: 10.1007/s10658-004-2134-z
Keywords
hormones; microarray; pathogen; root; transcription factor
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The root knot nematode Meloidogyne javanicaa plant parasite that is an agricultural pest, establishes and maintains a permanent feeding site within plant roots. In order to gain a broad view of gene expression in nematode feeding sites during the compatible response of tomato to root knot nematodes, we used the tomato spotted microarray chip, followed by real-time reverse transcription polymerase chain reaction (RT-PCR) validation. Cluster analysis and determination of the amplitude of gene expression suggested that the plant response at 5 days post-inoculation was not as vigorous as that at 10 days post-inoculation. In addition, by filtering the data for genes that were significantly up- or down-regulated during the interaction, we identified both the quantitative and qualitative differences between the two time points. Our microarray experiments demonstrated significant changes in the steady-state levels of transcripts of several functional categories, including pathogenesis-related genes, hormone-associated genes and development-associated transcription factors.
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