Measurement of root and leaf osmotic potential using the vapor-pressure osmometer

The vapor-pressure osmometer (VPO) is used to measure osmotic potential (psi(pi)) in plant water relations. The VPO was designed for 10 muL solution samples but its performance can be extended to suit smaller volumes and actual tissue samples. Measurement of psi(pi) by the VPO was compared to end-window (EW) psychrometry on known saline solutions and tissue samples varying from 10 to 2 muL. The VPO was a more sensitive instrument compared to the small EW psychrometer when using 10 muL of solution in the standard dish. Volumes varying from 3 to 10 muL could be used with reasonable accuracy and precision if a thinner disk (to ensure saturation) or smaller dish was used. Small samples such as root tips required a suitably small volume chamber to reduce effects of lowering psi(pi). For leaves from a field study, variability in plant replicates was greater than VPO sensitivity, so precision was comparable to using psychrometers. For direct measurement of water potential, lengthy equilibration times made the VPO an unsuitable method for measuring multiple samples. The VPO could be used for both small solution volumes and direct measurement of psi(pi) in tissue. (C) 2004 Elsevier B.V. All rights reserved.