Journal
GLYCOBIOLOGY
Volume 15, Issue 2, Pages 1C-6CPublisher
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwi007
Keywords
angiogenesis; endothelium; growth factor receptor; heparin; VEGF
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Vascular endothelial growth factor ( VEGF) represents a target for antiangiogenic therapies in a wide spectrum of diseases, including cancer. As a novel strategy to generate nonanticoagulant antiangiogenic substances exploiting binding to VEGF while preventing receptor engagement, we assessed the VEGF-antagonist activity of a low-molecular-weight (LMW) compound (ST2184, Mw = 5800) generated by depolymerization of an undersulfated glycol-split heparin derivative. The parental compound was obtained by introducing regular sulfation gaps along the prevalently N-sulfated heparin regions, followed by glycol-splitting of all nonsulfated uronic acid residues (similar to 50% of total uronic acid residues). ST2184 was endowed with a negligible anticoagulant activity after S. C. injection in mice. ST2184 binds VEGF(165) as evaluated by its capacity to retard I-125-VEGF(165) electrophoretic migration in a gel mobility shift assay and to prevent VEGF(165) interaction with heparin immobilized onto a BIAcore sensor chip. Unlike heparin, ST2184 was unable to present I-125- VEGF(165) to its high- affinity receptors in endothelial cells and inhibited VEGF(165)- induced neovascularization in the chick embryo chorioallantoic membrane. Undersulfated, LMW glycol-split heparins may therefore provide the basis for the design of novel nonanticoagulant angiostatic compounds.
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