Journal
INFECTION AND IMMUNITY
Volume 73, Issue 2, Pages 1226-1231Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.73.2.1226-1231.2005
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Funding
- NIAID NIH HHS [R15 AI047801, 1R15 AI 47801-01A2] Funding Source: Medline
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A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to D-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.
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