Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 7, Issue 2, Pages 177-182Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2004.12.004
Keywords
silica/gold/silver colloids; electrochemical behavior; diphtheria; immunoassay
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electrochemical behavior of a diphtheria immunosensor modified with nano-size conductor and semiconductor, as a mode of silica/gold/silver nanoparticles, has been investigated. Potentiometric immunosensor, cyclic voltammetry, and electrochemical impedance techniques were used to investigate the immobilization of diphtheria antibody on silicalgold/silver colloids. In the impedance spectroscopic study, an obvious difference of the electron transfer resistance between the silica-colloid modified electrode and the gold/silver-colloid modified electrodes was observed. The cyclic voltammogram tends to be more irreversible on silica colloids. Subsequently, using the silica colloid-modified immunosensor by potentiometry as a mode, it is found that the resulting immunosensor exhibited fast potentiometric response (<3 min), high sensitivity and good reproducibility. Moreover, analytical results of several specimens obtained using the silica colloid-modified immunosensor are in satisfactory agreement with those given by the ELISA method, implying a promising alternative approach for detecting diphtheria antigen in the clinical diagnosis. (C) 2004 Elsevier B.V. All rights reserved.
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