Journal
GENE EXPRESSION PATTERNS
Volume 5, Issue 3, Pages 363-369Publisher
ELSEVIER
DOI: 10.1016/j.modgep.2004.09.011
Keywords
zebrafish; cyp26b1; embryo; development; hindbrain; rhombomere; retinoid signaling; pectoral fin; retinoic acid; cytochrome p450
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Funding
- NICHD NIH HHS [HD39948] Funding Source: Medline
- NIEHS NIH HHS [ES11375] Funding Source: Medline
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We have cloned the zebrafish ortholog of the mammalian cyp26b1 gene. The predicted zebrafish cyp26b1 protein shares greater than 73% identity with mammalian homologues. cDNA transfection assays showed that like human cyp26b1, zebrafish cyp26b1 is involved in limiting the activity of retinoic acid. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of embryonic RNAs suggested that no maternal cyp26b1 message is detectable. Zygotic cyp26b1 message could be detected at 75% epiboly by RT-PCR and localized to presumptive rhombomere 3 and rhombomere 4 at the early two-somite (2S) stage (10.5 hpf. hour post fertilization) by whole mount in situ hybridization. As development proceeds expression expands anteriorly to include rhombomere 2 at the 10S stage (14hpf). By 14S (16hpf) expression in the hindbrain has also expanded posteriorly and encompasses rhombomere 2 through rhombomere 6. At later stages, 24 through 48 hpf, additional expression was found in the eyes, diencephalon, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia. (C) 2004 Elsevier B.V. All rights reserved.
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