Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 381, Issue 4, Pages 833-838Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-004-2972-8
Keywords
gold nanoparticle; electrochemical dna sensing; nanoparticle amplification; AFM
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A hybridization signal-amplified method based on a gold nanoparticle-supported DNA sequence for electrochemical DNA sensing has been investigated by cyclic voltammetry, differential-pulse voltammetry, and atomic-force microscopy (AFM). Quantitative analysis showed that the peak current increment (DeltaIp) is linearly dependant on the concentration of the gold nanoparticle-supported DNA sequence Au2 over the range 0.51-8.58 pmol L-1. AFM results indicated that the extent of surface hybridization was dependent on the concentration of the gold-nanoparticle-supported DNA sequence. Moreover, a new pair of peaks, which might arise from the special configuration of the gold-nanoparticle-supported DNA sequence, appeared in the cyclic voltammogram after hybridization. Although quite sensitive, this DNA sensing surface was not easily regenerated, so this kind of amplified method was suitable for disposable DNA sensors and chip-based gene diagnosis sensors.
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