Study of optical properties and biocompatibility of DLC films characterized by sp3 bonds

Optical and biomedical properties of diamond-like carbon (DLC) films of various sp(2), sp(3) bonds were studied. The layers were prepared by pulsed laser deposition (PLD) for laser energy densities from 4 J cm(-2) to 14 J cm(-2). The percentage of sp(2) and sp(3) bonds was calculated using X-ray photoelectron spectroscopy (XPS). In dependence on density the films contained up to 70 % of sp(3) bonds. Optical properties were measured using spectroscopic ellipsometry in region from 250 nm to 1000 nm (n=2.6-2.7; k=0.07-0.25) and by transmission measurement (from 200 nm to 1100 nm). The adhesion and growth of human fibroblasts and keratinocytes of DLC films were tested in vitro.