Journal
CELLULAR AND MOLECULAR LIFE SCIENCES
Volume 62, Issue 3, Pages 377-385Publisher
SPRINGER BASEL AG
DOI: 10.1007/s00018-004-4461-9
Keywords
chromogranin A; chromaffin secretory granule; catestatin; antimicrobial peptide; innate immunity; neuroimmunology
Categories
Ask authors/readers for more resources
Catestatin (bCGA(344-364)), an endogenous peptide of bovine chromogranin A, was initially characterized for its effect on the inhibition of catecholamine release from chromaffin cells. Catestatin and its active domain (bCGA(344-358)) were identified in chromaffin cells and in secretion medium. The present study identified a potent antimicrobial activity of bCGA(344-358) in the low-micromolar range against bacteria, fungi and yeasts, without showing any haemolytic activity. Confocal laser microscopy demonstrated penetration of the rhodaminated peptide into the cell membranes of fungi and yeasts and its intracellular accumulation. Time-lapse videomicroscopy showed arrest of fungal growth upon penetration of the labelled peptide into a fungal filament. We identified several catestatin-containing fragments in the stimulated secretion medium of human polymorphonuclear neutrophils, suggesting the N-terminal sequence of catestatin (bCGA344-358) (named cateslytin) as a novel component of innate immunity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available