Journal
PROCESS BIOCHEMISTRY
Volume 40, Issue 2, Pages 747-751Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2004.01.041
Keywords
sorbitol; Zymomonas mobilis sugar cane molasses; response-surface methodology
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Sorbitol production by Zymomonas mobilis in sugar cane molasses was investigated by batch fermentation. The effects of the mean sugar concentration, temperature, agitation, and culture time were studied simultaneously by factorial analysis. Maximum sorbitol production was determined using a second-order central composite design and analyzed by the superficial response method. Response-surface methodology analysis showed that the best conditions for sorbitol production were 300 g/l total reducing sugars (TRS) in the culture medium, 36 h at 35degreesC fermentation time. (C) 2004 Elsevier Ltd. All rights reserved.
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