In vivo antioxidant activity of carotenoids from Dunaliella salina -: a green microalga

Dunaliella salina a green marine alga is known for its carotenoid accumulation, having various applications in the health and nutritional products. The purpose of present study was to evaluate the ability of D. salina algal powder extract to protect against oxidative stress In vivo using animal models. Treatment of albino Wistar strain rats with 125 mug/kg and 250 mug/kg b.w. showed significant protection when compared to toxin treated (CCl4) group. Since beta-carotene is major constituent of Dunaliella the results were also compared with group treated with 250 mug/kg b.w (p.o.) synthetic all trans beta-carotene. Treatment of CCl4 at dose of 2.0 g /kg b.w decreased the activities of various antioxidant enzymes like catalase, superoxide dismutase (SOD) and peroxidase by 45.9%, 56% and 54% respectively compared to control group and lipid peroxidation value increased. nearly 2 folds. Pretreatment of rats with 125 mug carotenoid followed by CCl4 treatment caused restoration of catalase, SOD and peroxidase by 25.24%, 23.75 and 61.15% respectively as compared to control. The group treated with 250 mug/kg has shown the. restoration of 53.5%, 57.7 and, 90.64% of catalase, SOD and peroxidase, respectively. This group has shown 75.0% restoration of peroxidation compared to control group of animals. The above enzyme activities were not significantly restored in group treated with synthetic all trans beta-carotene, which showed 7.5%, 23.8% restore in catalase and peroxidase content. The level of superoxide dismutase remained same and lipid peroxidation value decreased only by 23% in synthetic all trans beta-carotene treated group in comparison with control group. These results clearly indicate the beneficial effect of algal carotenoid compared to synthetic carotene as antioxidant. Owing to this property, the algae Dunaliella can be further extended to exploit, its possible application for various health benefits as nutraceuticals and food additive. (C) 2004 Elsevier Inc. All rights reserved.