Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 815, Issue 1-2, Pages 3-9Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2004.10.030
Keywords
comparative proteomics; two-dimensional electrophoresis; shotgun peptide sequencing
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Two-dimensional electrophoresis (2-DE) and shotgun peptide sequencing are the two major technologies to compare the expression profile of proteins, which is also referred to as comparative proteomics or quantitative proteomics. Although the methodologies, such as difference gel electrophoresis for 2-DE and isotope-coded affinity tags for shotgun peptide sequencing, have made rapid progress, these two approaches have their own strengths and weaknesses. Therefore, the combination of the two methodologies is beneficial for the purpose of better comparative proteomics, especially in comprehensive coverage of the proteome and protein information such as post-translational modifications. (C) 2004 Elsevier B.V. All rights reserved.
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