Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 53, Issue 3, Pages 502-507Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf049224h
Keywords
milk; heat treatment; radial immunodiffusion; front-face fluorescence; intrinsic fluorophores; multivariate analysis
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Emission and excitation spectra of intrinsic fluorophores present. in milk were used to evaluate changes in milk following thermal treatments in the 57-72 degreesC temperature range from 0.5 min up to 30 min. Alternatively, the concentrations of native alkaline phosphatase, lactoferrin, immunoglobulin G, bovine serum albumin, beta-lactoglobulin, and alpha-lactalbumin were determined in the same samples by enzymatic and immunochemical techniques. As principal component analysis applied to the normalized fluorescence spectra successfully discriminated different milk samples according to the temperature and time of thermal treatment, principal component regression was applied to predict the amounts of the native proteins investigated using fluorescence data. The results showed strong correlations between measured and predicted data for alkaline phosphatase and beta-lactoglobulin. This study has demonstrated that front-face fluorescence spectroscopy has a promising potential to become a rapid and nondestructive analytical technique for the evaluation of physicochemical changes in milk induced by low thermal treatment.
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