Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 103, Issue 3-4, Pages 223-233Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2004.09.027
Keywords
non-viral peptide vector; CTLA-4; systemic lupus erythematosus; costimulation; dog
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Although viral vectors are commonly used for therapeutic acne delivery their applications are limited due to their specific cell membrane receptor-inediated infection and host immune response. In the present study, we constructed a non-viral peptide vector and applied it in the treatment of experimentally induced systemic lupus erythematosus-like disease in dogs. For therapeutic gene construction, the extracellular domain of canine CTLA-4, and the CH2-CH3 domains of canine immunoglobulin alpha constant region were inserted between the cytomegalovirus promoter and poly-adenylation sequence of bovine growth hormone. The constructed therapeutic gene was ligated to the non-viral synthetic peptide vector and was applied to systemic lupus erythematosus-like disease induced dogs. After gene therapy, clinical signs of systemic lupus erythematosus were reduced dramatically: the anti-nuclear antibody titers and urine protein/creatinine ratios were recovered to normal values, and the skin regained its normal histological features. The peptide vector did not show either tissue specific tropism or host induced immune response. (C) 2004 Elsevier B.V. All rights reserved.
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