Journal
FEBS LETTERS
Volume 579, Issue 5, Pages 1243-1248Publisher
WILEY
DOI: 10.1016/j.febslet.2005.01.018
Keywords
phosphatidylinositol 3-kinase; phosphatidylinositol(3)phosphate; reactive oxygen species; gravitropism; maize; Arabidopsis thaliana
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We recently reported that production of reactive oxygen species (ROS) is essential for auxin-induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3-kinase and its product, PtdIns(3)P, in auxin-mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3-kinase activity, blocked auxin-mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA-induced ROS accumulation. These results indicate that activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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