Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 141, Issue 2, Pages 219-222Publisher
ELSEVIER
DOI: 10.1016/j.jneumeth.2004.06.010
Keywords
thiobarbituric acid-reactive substances; oxidative stress; antioxidants; vitamin E; nervous system; neurodegeneration
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Oxidative stress is a pivotal factor in neuronal degeneration. A simple method to quantify oxidative damage in culture and in situ is therefore important for studies of neurodegeneration. We present herein modifications of the standard assay for thiobarbituric acid-reactive substances (TBARs) for analyses of both cell cultures and brain tissue homogenates. Since the TBAR assay measures end-point oxidative damage, it is useful to assess the overall impact of oxidative stress-inducing and neuroprotective agents; interpretation is not potentially confounded by the presence or absence of transient products of oxidative damage. (C) 2004 Published by Elsevier B.V.
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