Journal
GENES & DEVELOPMENT
Volume 19, Issue 4, Pages 445-452Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1267905
Keywords
Drosophila C virus; Drosophila; ribosome; translation; picornavirus; dicistroviridae
Categories
Funding
- NIAID NIH HHS [R01 AI051365, AI10532, T32 AI007328, AI051365, F32 AI010532] Funding Source: Medline
- NIGMS NIH HHS [GM55979, R01 GM055979] Funding Source: Medline
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The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.
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