Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 816, Issue 1-2, Pages 107-112Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2004.11.026
Keywords
alcohol biomarker; ethanol; 5-hydroxytryptophol glucuronide; LC-MS
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5-Hydroxytryptophol glucuronide (GTOL) is the major excretion form of 5-hydroxytryptophol (5-HTOL), a minor serotonin metabolite under normal conditions. Because the concentration of 5-HTOL is markedly increased following consumption of alcohol, measurement of 5-HTOL is used as a sensitive biomarker for detection of recent alcohol intake. This study describes the development and evaluation of a liquid chromatography-electrospray ionization mass spectrometry (LC-MS) procedure for direct quantification of GTOL in human urine. Deuterium labelled GTOL (GTOL-H-2(4)) was used as internal standard. GTOL was isolated from urine by solid-phase extraction on a C-18 cartridge prior to injection onto a gradient eluted Hypurity C-18 reversed-phase HPLC column. The detection limit of the method was 2.0 nmol/L and the measuring range 6-8500 nmol/L. The intra- and inter-assay coefficients of variation were <3.5% (n = 10) and <6.0% (n = 9), respectively. The new LC-MS method was highly correlated with an established GC-MS method for urinary 5-HTOL (r(2) = 0.99, it = 70; mean 5-HTOL/GTOL ratio = 1.10). This is the first direct assay for quantification of GTOL in urine. The method is suitable for routine application. (C) 2004 Elsevier B.V. All rights reserved.
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