Journal
BIOTECHNOLOGY LETTERS
Volume 27, Issue 6, Pages 417-422Publisher
SPRINGER
DOI: 10.1007/s10529-005-1776-z
Keywords
biotransformation; contact angles; surface properties; Yarrowia lipolytica; zeta potential
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The surface of the lipid-degrading yeast, Yarrowia lipolytica, was characterized by contact angle and zeta potential (zeta) measurements. The cells were mainly hydrophilic with a negative charge that was only affected from pH 2 to 4. To study the effects of the surface charges on the biotransformation of methyl ricinoleate into the aroma compound, gamma-decalactone, the gamma values of the substrate droplets were modified by adding a cationic surfactant into the medium at concentrations that did not diminish cell viability: the adhesion of the lipid substrate to the cells was increased but not the overall performance of the process, therefore the adhesion is not the rate limiting here. Our methodology offers interesting perspectives for further applications.
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