Nitrogen nutrition and isotopic discrimination in tropical ectomycorrhizal fungi

It is known that many ectomycorrhizal (EcM) fungi are able to utilise complex organic sources of nitrogen. Two hypotheses were tested using isolates of tropical EcM fungi grown in vitro: (i) EcM fungi isolated from mineral soils of tropical rain forests are less able to utilise organic sources of nitrogen than mineral sources; and (ii) nitrogen isotope discrimination patterns follow those of the nitrogen source utilised. Pisolithus albus and Tomentella sp. represented tropical EcM fungi and they were grown along with Thelephora terrestris. All three species were able to utilise bovine serum albumen as a nitrogen source and P albus produced the greatest biomass on this source of nitrogen. Nitrate was generally less well utilised than ammonium although all three species were able to grow on this nitrogen source. The nitrogen source which led to the greatest biomass also led to the highest fungal nitrogen concentration in P. albus and Tomentella sp., but not T terrestris. All three species discriminated against N-15 when grown on BSA and NO3 but there were interspecific differences in isotope discrimination when grown on NH4. From a limited number of isolates, it was found that EcM fungi from tropical soils utilise protein nitrogen as well as mineral nitrogen and there can be considerable nitrogen isotope discrimination during the utilisation of all these nitrogen sources. (c) 2004 Elsevier SAS. All rights reserved.