Journal
DEVELOPMENTAL DYNAMICS
Volume 232, Issue 3, Pages 733-737Publisher
WILEY
DOI: 10.1002/dvdy.20269
Keywords
Drosophila development; RNA stability; mRNA degradation; ribonucleases; gene regulation; Dis3; Rrp44p; exosome
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Funding
- Biotechnology and Biological Sciences Research Council [BB/C005163/1] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/C005163/1] Funding Source: researchfish
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The major 3'-5' pathway of RNA degradation in eukaryotic cells involves the exosome, which is a multiprotein complex of exoribonucleases. The exoribonucleases within this complex are highly conserved and are closely related to prokaryotic ribonucleases. We have identified and characterised the expression pattern of Drosophila tazman (taz), a component of the exosome which is closely related to Escherichia coli RNaseR and yeast Rrp44p. The tazman transcripts are differentially expressed during development, with maximum expression levels in 6-8 hr embryos. In situ hybridisation and immunolocalisation experiments show that tazman transcripts and protein are maternally derived, and are expressed ubiquitously throughout the embryo, with high levels in germ band and head structures. Differential expression of TAZ is likely to reflect changes in the activity of the 3'-5' mRNA turnover pathway which could have a major impact of the expression of target RNAs. (C) 2005 Wiley-Liss, Inc.
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