Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 12, Issue 3, Pages 270-273Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb905
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Resuscitation- promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)- rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.
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