Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 381, Issue 6, Pages 1130-1137Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-004-3028-9
Keywords
carbohydrates; oligosaccharides; reductive amination; mass spectrometry; NaBH(OAc)3; derivatization
Funding
- NCRR NIH HHS [1 P20 RR17708-01.] Funding Source: Medline
Ask authors/readers for more resources
An improved protocol for reductive amination of carbohydrates is developed. This derivatization facilitates the detection of oligosaccharides in HPLC-UV and mass spectrometric applications by enhancing the signal of the carbohydrates. In this study, reductive amination was achieved using NaBH(OAc)(3). This reducing agent is an attractive alternative to the toxic, but extensively used reducing agent, NaBH3CN. Several types of carbohydrates were successfully derivatized using NaBH(OAc)(3), and the results obtained from this protocol were compared with those obtained with NaBH3CN. Both reducing agents were equally effective in side-by-side analysis. Two purification strategies (purification by zip-tip and HPLC) were implemented and the instrumental limit of detection of each method was compared. The detection limit was similar to 1,000 times lower when the purification was done using HPLC, compared to using the zip-tip. Since the derivatization by-products in this protocol are not toxic, MS analysis also could also be performed directly, without purification. The MS/MS data of derivatized and underivatized oligosaccharides were acquired as well. The derivatized oligosaccharides produce more easily interpretable product ions than underivatized oligosaccharides.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available