Journal
GENETICS AND MOLECULAR BIOLOGY
Volume 28, Issue 1, Pages 150-151Publisher
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572005000100025
Keywords
phage screening; shortcut for Benton & Davis technique; single agar-plate/nylon filter
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A simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.
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