Journal
ECOLOGICAL RESEARCH
Volume 20, Issue 2, Pages 233-238Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s11284-004-0018-5
Keywords
revegetation; genetic differentiation; chloroplast DNA (cpDNA); amplified fragment length polymorphisms (AFLPs); PCR-RFLP
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We investigated the genetic structure of Miscanthus sinensis ssp. condensatus on Miyake Island, which was devastated by a volcanic eruption in 2000, by amplified fragment length polymorphisms (AFLPs) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for chloroplast DNA (cpDNA) variation, to develop recommendations for the revegetation of devastated sites. Genetic differentiation among populations was significant, and five populations were classified into three regional groups. The aspect ratios of leaf-blades varied significantly among populations, but both geographical proximity and morphological similarities did not precisely reflect genetic similarities. In the airport population, we found a rare haplotype that may have been transmitted from outside the island. These findings will assist the revegetation of the island.
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