Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 99, Issue 3, Pages 737-746Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2004.12.006
Keywords
dGTP; 8-Oxo-dGTP; Ni(II); binary complexes; ternary complexes; reactive oxygen species
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The formation of binary Ni(II) complexes with 2'-deoxyguanosine 5'-triphosphate (dGTP (1), L) as well as ternary complexes thereof with L-histidine (His, A) was studied with the use of potentiometry and electronic absorption spectroscopy. In the binary and ternary systems, the complexes with stoichiometries NiH2L-, NiHL2-, NiL3- and NiHLA'-, NiH(2)LA(2-), NiLA(4-) respectively, were detected. The ternary complexes are very stable at pH 7.4 and thus may constitute biologically relevant Ni(II) carriers in the cell. In the presence of hydrogen peroxide, the binary and ternary systems both generate hydroxyl radical-like species and undergo dGTP degradation with the formation of the 8-oxo-dGTP intermediate. The latter, along with dGTP complexation and degradation, may lead to mutagenesis and carcinogenesis due to base-mispairing properties of 8-oxoguanine and the disturbance in the physiological balance among the four canonical triphosphodeoxynucleotide substrates for DNA synthesis. (C) 2004 Elsevier Inc. All rights reserved.
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