Journal
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volume 449, Issue 6, Pages 564-572Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00424-004-1360-8
Keywords
ion channel; microfabrication; micromolding; PDMS
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Funding
- NIBIB NIH HHS [EB-002020] Funding Source: Medline
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We present a new technique for fabricating planar patch electrodes in the laboratory. Planar electrodes are micromolded using a micron-sized stream of air to define an aperture in the silicone elastomer, polydimethylsiloxane (PDMS). We have previously demonstrated that planar PDMS electrodes make excellent patch electrodes after surface modification. We demonstrate single-channel measurements of the rSlo channel in Xenopus oocytes and whole-cell measurements in CHO and RBL mammalian cell lines, using planar PDMS electrodes.
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