Journal
JOURNAL OF PROTEOME RESEARCH
Volume 4, Issue 2, Pages 424-434Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr049804u
Keywords
chemical-enzymatic; phosphorylation; 2-aminoethanethiol; isotope dilution mass spectrometry; phosphoproteins; phosphopeptides; quantification
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Funding
- NCI NIH HHS [P30 CA015083-319015, P30 CA015083] Funding Source: Medline
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A novel chemical-enzymatic approach was developed to facilitate identification of phosphorylation sites in isolated phosphoproteins. ESI-TOF mass spectrometry was used to characterize products from the chemical-enzymatic cleavage of specific phosphorylation sites in bovine alpha-S1 casein and synthetic phosphopeptides containing substitutions at a single phosphorylation site. Further refinements to this approach for identification of protein phosphorylation sites and its utility for the quantification of phosphopeptides by isotope-dilution mass spectrometry are presented.
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