Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 77, Issue 1, Pages 77-94Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/S0001-37652005000100006
Keywords
Trypanosoma cruzi; cellular invasion; amastigotes; trypomastigotes; parasitophlorous vacuole escape; trafficking; Coxiella burnetii; phylogenetic lineages
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Trypanosoma cruzi, the etiological agent of Chagas' disease. occurs as different strains or isolates that may be grouped in two major phylogenetic lineages: T. cruzi I, associated with the sylvatic cycle and T cruzi H, linked to the human disease. In the mammalian host the parasite has to invade cells and many studies implicated the flagellated trypomastigotes in this process. Several parasite surface components and some of host cell receptors with which they interact have been identified. Our work focused on how amastigotes. usually found growing in the cytoplasm, can invade mammalian cells, with infectivities comparable to that of trypomastigotes. We found differences in cellular responses induced by amastigotes and trypomastigotes regarding cytoskeletal components and actin-rich projections. Extracellularly generated amastigotes of T cruzi I strains may display greater infectivity than metacyclic trypomastigotes towards cultured cell fines as well as target cells that have modified expression of different classes of cellular components. Cultured host cells harboring the bacterium Coxiella burnetii allowed us to gain new insights into the trafficking properties of the different infective forms of T cruzi, disclosing unexpected requirements for the parasite to transit between the parasitophorous vacuole to its final destination in the host cell cytoplasm.
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