Journal
AMINO ACIDS
Volume 28, Issue 2, Pages 183-191Publisher
SPRINGER WIEN
DOI: 10.1007/s00726-005-0166-0
Keywords
nefopam; voltage sensitive calcium channels; excitotoxicity; cGMP; cultured cerebellar neurons
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Nefopam hydrochloride is a potent non sedative benzoxazocine analgesic that possesses a profile distinct from that of anti- inflammatory drugs. Previous evidence suggested a central action of nefopam but the detailed mechanism remains unclear. We have investigated the actions of nefopam on voltage sensitive calcium channels and calcium-mediated pathways. We found that nefopam prevented N-methyl-D-aspartate( NMDA)- mediated excitotoxicity following stimulation of L- type voltage sensitive calcium channels by the specific agonist BayK8644. Nefopam protection was concentration-dependent. 47 mu M nefopam provided 50% protection while full neuroprotection was achieved at 100 mu M nefopam. Neuroprotection was associated with a 73% reduction in the BayK8644-induced increase in intracellular calcium concentration. Nefopam also inhibited intracellular cGMP formation following BayK8644 in a concentration-dependent manner, 100 mu M nefopam providing full inhibition of cGMP synthesis and 58 mu M allowing 50% cGMP formation. Nefopam reduced NMDA receptor-mediated cGMP formation resulting from the release of glutamate following activation of channels by BayK8644. Finally, we also showed that nefopam effectively reduced cGMP formation following stimulation of cultures with domoic acid, while not providing neuroprotection against domoic acid. Thus, the novel action of nefopam we report here may be important both for its central analgesic effects and for its potential therapeutic use in neurological and neuropsychiatric disorders involving an excessive glutamate release.
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