Journal
PLANT CELL REPORTS
Volume 23, Issue 10-11, Pages 721-726Publisher
SPRINGER
DOI: 10.1007/s00299-004-0876-x
Keywords
FLP-FRT; Cre-lox; site-specific recombination; rice transformation
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To develop an FLP-FRT recombination system( derived from 2 m plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the beta-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system ( derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.
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