Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 381, Issue 6, Pages 1179-1185Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-004-3032-0
Keywords
biosensor; horseradish peroxidase; self-assemble; silver nanoparticles; AFM
Ask authors/readers for more resources
A third-generation hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) on a gold electrode modified with silver nanoparticles. A freshly-cleaned gold electrode was first immersed in a cysteamine-ethanol solution, and then silver nanoparticles were immobilized on the cysteamine monolayer, and finally HRP was adsorbed onto the surfaces of the silver nanoparticles. This self-assemble process was examined via atomic force microscopy (AFM). The immobilized horseradish peroxidase exhibited an excellent electrocatalytic response toward the reduction of hydrogen peroxide. The linear range of the biosensor was 3.3 mu M to 9.4 mM, and the detection limit was estimated to be 0.78 mu M. Moreover, the biosensor exhibited a fast response, high sensitivity, good reproducibility, and long-term stability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available