Journal
FOOD CHEMISTRY
Volume 90, Issue 1-2, Pages 157-167Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2004.03.049
Keywords
Hypericuin perfortum; phenolics; flavonoids; antioxidant activity; lipid peroxidation inhibition
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The antioxidant potentials of a total ethanolic extract of Hypericum perforatum. (TE) and fractions were evaluated and correlated with their phenolic contents. The extracts were fully characterised by HPLC-DAD-MS-MS. Kaempferol 3-rutinoside and rutin-acetyl were identified for the first time in TE extracts. The free radical-scavenging properties of TE (EC50 = 21 mug dwb/ml) and fractions were studied using DPPH. Fractions containing flavonoids and/or caffeoylquinic acids were found to be the main contributors to the free radical-scavenging activity of the TE. Lipid peroxidation, induced with ascorbate/Fe2+, was significantly reduced in the presence of the TE (EC50 = 26 mug dwb/ml) and fractions containing flavonoids and/or caffeoylquinic acids. The fraction containing flavonoid aglycones was found to be responsible for a major part of the TE protection against lipid peroxidation. Hypericins and hyperforins made no significant contribution's to the antioxidant properties of TE. Human consumption of H. perforation extract or fractions could be beneficial. (C) 2004 Elsevier Ltd. All rights reserved.
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