Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 52, Issue 1, Pages 129-137Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsec.2004.10.012
Keywords
Halobacterium salinarium; Halorubrum distributum; Brevibacterium antiquum; magnesium phosphate; polyphosphate; phosphate uptake
Categories
Ask authors/readers for more resources
Stationary phase cells of the halophilic archaea Halobacterium salinarium and Halorubrum distributum, growing at 3-4 M NaCl, and of the halotolerant bacterium Brevibacterium antiquum, growing with and without 2.6 NaCl, took up similar to 90% of the phosphate from the culture media containing 2.3 and 11.5 mM phosphate. The uptake was blocked by the uncoupler FCCP. In B. antiquum, EDTA inhibited the phosphate uptake. The content of polyphosphates in the cells was significantly lower than the content of orthophosphate. At a high phosphate concentration, up to 80% of the phosphate taken up from the culture medium was accumulated as Mg2PO4OH center dot 4H(2)O in H. salinarium and H. distributum and as NH4MgPO4 center dot 6H(2)O in B. antiquum. Consolidation of the cytoplasm and enlargement of the nucleoid zone were observed in the cells during phosphate accumulation. At phosphate surplus, part of the H. salinarium and H. distributum cell population was lysed. The cells of B. antiquum were not lysed and phosphate crystals were observed in the cytoplasm. (c) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V.. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available