Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 102, Issue 22, Pages 9563-9575Publisher
SPRINGER
DOI: 10.1007/s00253-018-9359-9
Keywords
Fourier transform infrared spectroscopy; Hairy root culture; Methyl jasmonate; Phenolic compounds; Polygonum multiflorum
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Funding
- Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program - Ministry of Agriculture, Food and Rural Affairs (MAFRA) [315013-4]
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Hairy root cultures (HRCs) are characterized by fast and unlimited root growth, and they have greater genetic stability than other cultivation methods. HRCs are known to accumulate phytochemical levels comparable to those of intact plant. In this study, HRCs of Polygonum multiflorum were established from leaf explants infected with Agrobacterium rhizogenes strain KCCM 11879. Over 60% of the explants showed hairy root induction after 21days of cultivation on hormone-free MS (Murashige and Skoog Physiol Plant 15:473-479, 1962) medium; induced roots were confirmed by PCR using a rolC-specific primer. Of the six lines of HRCs selected for further analysis, line HR-01 performed best, producing a root biomass (105.2gL(-1) of FW, 9.7gL(-1) of DW), which is 10-fold higher than that of non-transgenic roots. The HR-01 line also showed a significant increase in its total phenolic content (26.64mgg(-1) DW), while non-transgenic roots accumulated 8.36mgg(-1) DW of total phenolic. The levels of phenolic compounds in the HRCs increased more than 2.5-fold following exposure to 50M methyl jasmonate for 5days. Fourier transform infrared (FT-IR) spectroscopic analysis of bioactive accumulation in P. multiflorum enabled discrimination between hairy root and adventitious root cultures. Thus, it is evident from this study that HRCs could be an attractive proposition for large-scale production of root biomass and secondary metabolites of P. multiflorum in bioreactors.
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