Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 98, Issue 22, Pages 9473-9481Publisher
SPRINGER
DOI: 10.1007/s00253-014-6088-6
Keywords
Chlorella sorokiniana; Fe; Lipid biosynthesis; qPCR; Genefishing
Categories
Funding
- Chinese Natural Science Foundation For Distinguished Group [50621063]
- Central South University [2010bsxt05]
- NSF EFRI [1332344]
- Emerging Frontiers & Multidisciplinary Activities
- Directorate For Engineering [1332344] Funding Source: National Science Foundation
Ask authors/readers for more resources
The effects of iron on the growth, lipid accumulation, and gene expression profiles of the limnetic Chlorella sorokiniana CCTCC M209220 under photoautotrophy were investigated. The addition of iron up to 10(-5) mol l(-l) increased final cell densities by nearly 2-fold at 2.3 x 10(7) cells/ml, growth rate by 2-fold, and the length of the exponential phase by 5 days as compared to unsupplemented controls while 10(-3) mol l(-1) iron was toxic. The lipid content increased from 12 % for unsupplemented cultures to 33 % at 10(-4) mol l(-1) iron while the highest overall lipid yield reached 179 mg l(-1). A genefishing and qPCR comparison between the C. sorokiniana at low and high iron levels indicated increases in the expression of several genes, including carbonic anhydrase involved in microalgal cell growth, as well as acc1 and choline transporter related to lipid synthesis. This study provides insights into changes in gene expression and metabolism that accompany iron supplementation to Chlorella as well as potential metabolic engineering targets for improving growth and lipid synthesis in microalgae.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available