Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 98, Issue 16, Pages 6991-7001Publisher
SPRINGER
DOI: 10.1007/s00253-014-5704-9
Keywords
Autotransporter; Surface display; gamma-Lactamase
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Funding
- State Key Laboratory of Microbial Resources, Institute of Microbiology, CAS
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At present, autotransporter protein mediated surface display has opened a new dimension in the development of whole-cell biocatalysts. Here, we report the identification of a novel autotransporter Xcc_Est from Xanthomonas campestris pv campestris 8004 by bioinformatic analysis and application of Xcc_Est as an anchoring motif for surface display of gamma-lactamase (Gla) from thermophilic archaeon Sulfolobus solfataricus P2 in Escherichia coli. The localization of gamma-lactamase in the cell envelope was monitored by Western blot, activity assay and flow cytometry analysis. Either the full-length or truncated Xcc_Est could efficiently transport gamma-lactamase to the cell surface. Compared with the free enzyme, the displayed gamma-lactamase exhibited optimum temperature of 30 A degrees C other than 90 A degrees C, with a substantial decrease of 60 A degrees C. Under the preparation system, the engineered E. coli with autodisplayed gamma-lactamase converted 100 g racemic vince lactam to produce 49.2 g (-) vince lactam at 30 A degrees C within 4 h. By using chiral HPLC, the ee value of the produced (-) vince lactam was determined to be 99.5 %. The whole-cell biocatalyst exhibited excellent stability under the operational conditions. Our results indicate that the E. coli with surface displayed gamma-lactamase is an efficient and economical whole cell biocatalyst for preparing the antiviral drug intermediate (-) vince lactam at mild temperature, eliminating expensive energy cost performed at high temperature.
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