Journal
PHYTOCHEMICAL ANALYSIS
Volume 16, Issue 2, Pages 120-126Publisher
WILEY
DOI: 10.1002/pca.830
Keywords
HPLC-SPE-NMR; structural identification lignans; Phyllanthus urinaria
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HPLC-SPE-NMR has been applied to the analysis of a number of structurally similar lignans present in Phyllanthus urinaria. A reversed-phase HPLC system, using a C. column eluted with tetrahydrofuran-water-methanol and detected at 225 nm was developed that gave good resolution for seven lignans from Phyllanthus urinaria. Coupling of this system with SPE-NMR provided clean H-1-NMR spectra for nine lignans present in 4 mg of a lignan-rich fraction, equivalent to ca. 1.0 g of dry plant material. The result demonstrates that HPLC-SPE-NMR is a very efficient and powerful too for the unambiguous identification of natural products that have been previously characterised. Copyright (c) 2005 John Wiley & Sons, Ltd.
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